Antiparallel - Describes parallel strands in DNA that run in opposite directions in terms of chemical polarity, Base - Nitrogenous substance that is a component of DNA nucleotides, Deoxyribose - Sugar that is a component of DNA nucleotides, DNA - Deoxyribonucleic acid: molecule that holds the genetic code in living organisms, DNA Polymerase - Enzyme that adds free nucleotides during replication, Double stranded helix - Three-dimensional shape of a DNA molecule, Gel electrophoresis - Laboratory method used to separate mixtures of DNA, RNA or proteins according to size, Heat-tolerant DNA polymerase - Enzyme from hot spring bacteria, used in PCR, Hydrogen bond - Weak chemical link joining complementary base pairs, Nucleotide - Component of DNA consisting of a deoxyribose sugar, a phosphate group and a nitrogenous base, Phosphate - Component of a DNA nucleotide, PCR - Method of amplifying sequences of DNA in vitro, Sugar-phosphate backbone - Strongly bonded strand of DNA, Target sequence - Complementary sequence of bases on the 3’ end of DNA to which primers attach, Template strand - DNA strand on which a complimentary copy is made, Primers - Short strands of nucleotides whichare complementary to specific target sequences at the two ends of the region of DNA to be amplified,

Higher 1.2 PCR Key Terms

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