Denaturing: 94 °C, DNA sample separated into two separate strands., Hydrogen bonds between the complementary DNA strands break., , Annealing: 55 °C, Short segments of single-stranded DNA, known as primers are added., Primers bind to the target DNA sequences (at 3' end) , , Extension: 72 °C, Taq polymerase, extending the primers, synthesising the new strands of DNA by adding nucleotides, New strand of ‘complementary DNA’ to each of the single template created., ,

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