1) Which statement about the transition state (enzyme-substrate) molecule is correct? a) Specificity of enzyme function depends on the concentration of substrate b) Substrate molecules bind non-covalently to the active site of the enzyme causing an allosteric change in the enzyme that lowers the activation energy of the reaction. c) Enzyme active sites provide the water molecules needed for dehydration synthesis by splitting water and attaching it to the substrates during covalent bond formation d) Substrate molecules bind non-covalently to the enzyme's active site and change the surrounding pH by forming hydrogen bonds 2) Predict the experimental treatment that would result in the highest overall rate of reaction for the given enzyme. a) Experiment in which temperature is kept at 32°C in a solution of pH 4. b) Experiment in which temperature is kept at 50°C in a solution of pH 8. c) Experiment in which temperature is kept at 15°C in a solution of pH 8. d) Experiment in which temperature is kept at 40°C in a solution of pH 11. 3) Choose the pair of terms that correctly completes this sentence: Catabolism is to anabolism as _________________ is to____________________. a) exergonic; endergonic b) free energy; activation energy c) exergonic; spontaneous d) endergonic; exergonic 4) Which of the following is not a form of potential energy in biology? a) ATP b) covalent bonds c) ionic bonds d) proton (H+) gradients 5) Simple structures work together to make more complex structures, and together their emergent properties help to define life on earth. What structure is considered the most basic unit of life that exhibits all the properties of life? a) a chloroplast b) a DNA molecule c) a mitochondria d) a cell e) an atom 6) Choose ALL prokaryotic cells listed from the illustration. a) a flu virus b) bacteria cell c) a protein d) human egg cell e) a plant cell f) an animal cell 7) Choose ALL items that that do not meet the requirements of being alive in the illustration. a) protein b) a mitochondria c) a lipid d) frog egg cell e) an atom f) a bacteria cell 8) Which of the following molecules is entirely hydrophobic? a) b) c) d) 9) The rate of an enzymatic reaction plateaus as substrate concentrations increase because - a) the activation energy of the active sites is too high. b) the high substrate concentrations are inhibitors of enzyme active sites. c) there are a limited number of the active sites present d) the substrate concentration reaches a point at which it cannot increase any further 10) A strand of DNA with the sequence 5'-GCC TTA-3' would have a complementary strand with the sequence: a) 5'-ACCTTA-3' b) 3'-AAAAAA-5' c) 5'-ATTCCG-3'. d) 5'-TAAGGC-3' 11) Choose all the true statements about enzymes. a) Enzymes lower the activation energy. b) Most enzymes bind a diversity of substrates. c) Enzymes are highly specific. d) Enzymes are lipids e) An enzyme changes shape when it binds to a substrate. f) An enzyme may orient substrates, induce strain, or temporarily add chemical groups. 12) The transport of a solute using the energy of a proton gradient is an example of ______________ energy. a) kinetic b) potential c) dark d) none of the above 13) The transport of protons to generate a proton gradient is an example of ____________________ energy. a) kinetic b) potential c) dark d) crazy 14) Mark all that are true of Steroid Hormones... a) are hydrophobic b) diffuse across a membrane c) bind to a receptor inside the cell d) polar e) bind to a receptor on the cell membrane f) make proteins 15) In what City does the majority of Stranger Things take place? a) b) c) d)

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