Cut 15 cylinders of tissue, each approximately 50mm long, on the chopping board use the scalpel to remove any periderm as this would stop osmosis., Place 30cm 3  distilled water or solution in each test tube. You must label each test tube. , Using the scalpel and forceps, ensure the ends of the cylinder are at 90 degrees to its length., Measure the length of the cylinder to the nearest mm or the mass to the nearest 0.01g., Using the forceps, place 3 cylinders into each boiling tube., Leave at room temperature for a minimum of 45 minutes, or overnight at 4 oC. , Gently blot the cylinders and re-measure the length or re-weigh the mass of the cylinders., Record your results in a table. , Plot the mean percentage change against the concentration of solution. , Estimate the solute potential of the tissue. .

AS Level WJEC Biology Osmosis Practical

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