Production of insulin is using Escherichia coli, ____ for insulin is extracted from beta cell of the Islet of Langerhans of ____ it is used as template to synthesis ____ by ____ and DNA polymerase. DNA containing the insulin gene is directly isolated from pancreatic cells. The cDNA/DNA is cut using ____ (such as EcoRI). To form DNA fragments including the one containing the required gene with sticky ends. (Note: Italic/underlined 'Escherichia coli'/ 'E.coli' 'Eco'RI)) Cloning vector / ____ from Escherichia coli is isolated. The plasmid is cut with the ____ restriction enzyme to produce linear plasmid with ____ sticky ends. cDNA / DNA fragment ____ into cut plasmid with aid of ____ to form ____ / plasmid. Recombinant DNA is transferred to Escherichia coli by ____. ____ is performed to select Escherichia coli with recombinant DNA. Escherichia coli with recombinant DNA undergoes cloning / ____ insulin gene will be expressed insulin is extracted from Escherichia coli. (Note: Italic/underlined 'Escherichia coli'/ 'E.coli' 'Eco'RI))

Discuss the process of producing human insulin through DNA recombinant technology using Escherichia coli

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